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ATCC
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ATCC
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ATCC
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ATCC
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Markstein Sichtec Medical
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Promega
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Markstein Sichtec Medical
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BestGene Inc
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Dragon Genomics
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Promega
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GenScript corporation
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Novacta Biosystems Limited
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Image Search Results
Journal: PLoS ONE
Article Title: A New In Vitro Strand Transfer Assay for Monitoring Bacterial Class 1 Integron Recombinase IntI1 Activity
doi: 10.1371/journal.pone.0001315
Figure Lengend Snippet: Reactions were performed for 90 min in the presence of purified IntI1 (5 or 10 pmoles), 0.1 to 0.2 pmoles of either pGEM-T- attI1 or pGEM-T- attC (p attI1 and p attC in the figure) and 0.1 pmoles free 5′ 32 P radiolabeled recombination sites under standard conditions described in . Products were loaded on 1% agarose gel and autoradiographied (A). F: free recombination sites, RP: recombination products. The recombination products were quantified and the percentage of recombination versus the amount of IntI in pmoles was plotted (B).
Article Snippet: The receptor plasmids pGEM-T- attI1 (called p attI1 ) and
Techniques: Purification, Agarose Gel Electrophoresis
Journal: PLoS ONE
Article Title: A New In Vitro Strand Transfer Assay for Monitoring Bacterial Class 1 Integron Recombinase IntI1 Activity
doi: 10.1371/journal.pone.0001315
Figure Lengend Snippet: Reactions were performed for 90 min in the presence of purified enzyme (5 pmoles), 0.1 pmoles of linear radiolabeled recombination sites attI1 or attC and 0.1 pmoles of pGEM-T- attI1 under standard conditions described in section. Products were loaded on 1% agarose gel and autoradiographied. F: free recombination sites, RP: recombination products.
Article Snippet: The receptor plasmids pGEM-T- attI1 (called p attI1 ) and
Techniques: Purification, Agarose Gel Electrophoresis
Journal: PLoS ONE
Article Title: A New In Vitro Strand Transfer Assay for Monitoring Bacterial Class 1 Integron Recombinase IntI1 Activity
doi: 10.1371/journal.pone.0001315
Figure Lengend Snippet: Reactions were performed for 90 min in the presence of purified enzyme (5 pmoles), 0.1 pmoles of linear radiolabeled double-stranded (ds) or single-stranded (bottom strand: ss bot, or top strand: ss top) recombination sites attI1 or attC and 0.1 pmoles of pGEM-T- attI1 or pGEM-T- attC under standard conditions described in section. Products were loaded on 1% agarose gel and autoradiographied. F: free recombination sites, RP: recombination products.
Article Snippet: The receptor plasmids pGEM-T- attI1 (called p attI1 ) and
Techniques: Purification, Agarose Gel Electrophoresis